Nano Letters-Mapping Mechanical Force Propagation through Biomolecular Complexes

Mapping Mechanical Force Propagation through Biomolecular Complexes

Constantin Schoeler†, Rafael C. Bernardi‡, Klara H. Malinowska†, Ellis Durner†, Wolfgang Ott†§, Edward A. Bayer∥, Klaus Schulten‡⊥, Michael A. Nash*†, and Hermann E. Gaub†
† Lehrstuhl für Angewandte Physik and Center for Nanoscience, Ludwig-Maximilians-Universität, 80799 Munich, Germany
‡ Theoretical and Computational Biophysics Group, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
§ Center for Integrated Protein Science Munich (CIPSM), University of Munich, 81377 Munich, Germany
∥ Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel
⊥ Department of Physics, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
Nano Lett., 2015, 15 (11), pp 7370–7376
DOI: 10.1021/acs.nanolett.5b02727
Publication Date (Web): August 11, 2015
Copyright © 2015 American Chemical Society
Single-molecule force spectroscopy with an atomic force microscope (AFM) and steered molecular dynamics (SMD) simulations revealed force propagation pathways through a mechanically ultrastable multidomain cellulosome protein complex.