JACS-Cellulosomal Scaffoldin Mechanics

Combining in Vitro and in Silico Single-Molecule Force Spectroscopy to Characterize and Tune Cellulosomal Scaffoldin Mechanics

Tobias Verdorfer†, Rafael C. Bernardi‡, Aylin Meinhold†, Wolfgang Ott†, Zaida Luthey-Schulten‡§, Michael A. Nash*∥⊥, and Hermann E. Gaub† 
† Lehrstuhl für Angewandte Physik and Center for Nanoscience, Ludwig-Maximilians-Universität, 80799 Munich, Germany
‡ Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
Department of Chemistry, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
Department of Chemistry, University of Basel, 4056 Basel, Switzerland
Department of Biosystems Science and Engineering, Swiss Federal Institute of Technology (ETH Zurich), 4058 Basel, Switzerland
J. Am. Chem. Soc., 2017, 139 (49), pp 17841–17852
DOI: 10.1021/jacs.7b07574
Publication Date (Web): October 23, 2017
Copyright © 2017 American Chemical Society

The force induced unfolding behavior of all cohesins from the scaffoldin ScaA was successfully characterized by combining in vitro and in silico single-molecule force spectroscopy.